Invitrogen™ Blimp-1 Monoclonal Antibody (3H2-E8)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA116874

Product Code. 11564412

Description

In Western Blot, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody does not immunoprecipitate.For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification; this antibody demonstrates nuclear staining. Suggested positive control: antigen standard for PRDM1 (transient overexpression lysate), P3X (murine plasmocytoma) cell lysates.

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

Specifications

• Antigen: Blimp-1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: O75626, Q60636
• Gene Symbols: PRDM1
• Immunogen: A fragment of mouse Blimp-1 corresponding to residues 199-409.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Pig
• Product Type: Antibody
• Isotype: IgG1

• Applications: ChIP Assay, ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 3H2-E8
• Conjugate: Unconjugated
• Gene: PRDM1
• Gene Alias: B lymphocyte-induced maturation protein 1; b2b1765Clo; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; Blimp1; BLIMP-1; B-lymphocyte-induced maturation protein 1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PR/SET domain 1; PRDI BF1; PRDI-BF1; PRDI-binding factor-1; Prdm1; RP1-134E15.1; ZNFPR1A1
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100154284, 12142, 639
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid