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Invitrogen™ Blasticidin S Deaminase Polyclonal Antibody

Product Code. 17876778
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Quantity:
100 μL
Unit Size:
100µL
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17876778 100 μL 100µL
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Product Code. 17876778 Supplier Invitrogen™ Supplier No. PA5143657

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Description

Rabbit Polyclonal Antibody

The antibody detects a 13 kDa* protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells.

Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Blasticidin S Deaminase
Applications Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 0.25 mg/mL
Conjugate Unconjugated
Formulation PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene BAD
Gene Accession No. Q61337
Gene Alias blasticidin; BSD
Host Species Rabbit
Immunogen A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase* from Aspergillus Terreus.
Purification Method Antigen affinity chromatography
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 12015
Target Species Fungi
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Liquid
Isotype IgG
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