Cytiva BioTrak™ MMP-2 Activity Assay

Product Code. 10301335

Brand: Cytiva RPN2631

Description

Provides a simple, specific and precise quantitative determination of active or pro MMP-2 in tissue culture supernatants, serum, plasma, urine, synovial fluid and tissue homogenates

• Specific for Matrix Metalloproteinase-2 (MMP-2)
• Either the endogenous (naturally occurring) or total (following activation using APMA) activity levels in samples can be detected
• Range and sensitivity equivalent to ELISA
• Non-radioactive coated microplate format for ease of use
• Measures active MMPs from a range of species, including human, mouse, rat, rabbit and porcine
• Applicable for complex biological samples such as serum, plasma, synovial fluids, tissue extracts, cell culture supernatants
• Flexible protocol to cover speed and sensitivity requirements

Specifications

• Antigen: MMP-2
• Assay Range: 0.75 ng/ml/ (0.19 ng/ml possible with longer incubation)-12 ng/ml (3 ng/ml possibile with longer incubation)
• Description: Matrix Metalloproteinase-2, Biotrak Assay
• Molecular Weight (g/mol): 66000
• Quantity: 96-wells
• Sample Volume: 100 μL
• Sensitivity: 0.5 ng/mL (short incubation), 190 pg/mL (longer incubation)
• Content And Storage: -15°C to -30°C
• For Use With (Application): ELISA
• Specificity: The assay recognises the pro and active forms of MMP-2. It does not cross-react with other MMPs or TIMPs. Cross-reactivity has also been shown with rabbit, porcine, rat and mouse samples. Both pro and active MMP-2/TIMP-2 complexes as well as active MMP-2/

Safety and Handling

Signal Word

• Danger

Hazard Category

• LONG-TERM AQUATIC HAZARD Chronic 3
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2
• Acute toxicity Category 4
• Acute toxicity Category 2
• Acute toxicity Category 1
• Specific target organ toxicity after repeated exposure Category 2
• ACUTE AQUATIC Acute 1
• LONG-TERM AQUATIC HAZARD Chronic 1

Hazard Statement

• H412-Harmful to aquatic life with long lasting effects.
• H319-Causes serious eye irritation.
• H315-Causes skin irritation.
• H302-Harmful if swallowed.
• H332-Harmful if inhaled.
• H300-Fatal if swallowed.
• H310-Fatal in contact with skin.
• H330-Fatal if inhaled.
• H373-May cause damage to organs.
• H400-Very toxic to aquatic life.
• H410-Very toxic to aquatic life with long lasting effects.

Precautionary Statement

• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P264-Wash thoroughly after handling.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P271-Use only outdoors or in a well-ventilated area.
• P270-Do not eat, drink or smoke when using this product.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P262-Do not get in eyes, on skin, or on clothing.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302-if on skin: .
• P405-Store locked up.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : 2-amino-2-(hydroxymethyl)propane-1, 3-diol hydrochloride, zinc chloride, trometamol, S-2444 substrat