Invitrogen™ beta Arrestin 1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA587369

Product Code. 16585871

Description

Positive Samples: A-549, Mouse spleen; Cellular Location: Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane, Nucleus, clathrin-coated pit, pseudopodium Immunogen sequence: LASSTLLREG ANREILGIIV SYKVKVKLVV SRGGLLGDLA SSDVAVELPF TLMHPKPKEE PPHREVPENE TPVDTNLIEL DTNDDDIVFE DFARQRLKGM K.

Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.

Specifications

• Antigen: beta Arrestin 1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ARRB1
• Gene Alias: 1200006I17Rik; ARB1; ARR1; ARRB1; ARRB1 protein; arrdc1; arrestin; arrestin 2; arrestin beta 1; arrestin beta-1; arrestin, beta 1; arrestin-2; AW208571; BARRES; beta-arrestin; beta-arrestin1; beta-arrestin-1; G430100A01Rik; Non-visual arrestin-2
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 109689, 25387, 408
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.63 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P29066, P49407, Q8BWG8
• Gene Symbols: ARRB1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-400 of human beta-arrestin1 (NP_0040322).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG