PeproTech beta-5 Defensin Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  PeproTech 500-P323BT-50UG

Product Code. 17805463

Description

AA Sequence of recombinant protein: GLDFSQPFPS GEFAVCESCK LGRGKCRKEC LENEKPDGNC RLNFLCCRQR I. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BD-5. Anti-Human BD-5-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hBD-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-5 (500-P323) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-5. Western Blot: To detect hBD-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P323BT-1MG will be provided as 2 x 500 μg

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, six human beta-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.

Specifications

• Antigen: beta-5 Defensin
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: DEFB105A
• Gene Symbols: DEFB105A, DEFB105B
• Immunogen: E.coli-derived Recombinant Human BD-5.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q8NG35
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 245908, 504180
• Content And Storage: -20°C
• Form: Lyophilized