PeproTech beta-2 Defensin Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Goat Polyclonal Antibody

Product Code	Brand	Quantity
17834583	PeproTech 500-P161GBT-25UG	25 μg
17833933	PeproTech 500-P161GBT-50UG	50 μg
17824283	Invitrogen 500-P161GBT-1MG	2 x 500 μg
30054599	PeproTech 500-P161GBT-500UG	500 μg

Description

AA Sequence of recombinant protein: GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human BD-2. Anti-Human BD-2-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hBD-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-2 (500-P161G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-2. Western Blot: To detect hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P161GBT-1MG will be provided as 2 x 500 μg

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, six human beta-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.

Specifications

• Antigen: beta-2 Defensin
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Goat
• Target Species: Human
• Gene Accession No.: O15263
• Gene ID (Entrez): 1673
• Immunogen: E.coli-derived Recombinant Human BD-2.
• Purification Method: Antigen affinity chromatography
• Gene: DEFB4A

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: BD-2; beta defensin 2; beta defensin-2; Beta-defensin 2; Beta-defensin 4A; DEFB102; DEFB2; DEFB-2; DEFB4; DEFB4A; DEFB4B; Defensin beta 2; defensin beta 4A; Defensin beta2; defensin, beta 2; defensin, beta 4; defensin, beta 4A; Defensin-beta2; HBD-2; SAP1; Skin-antimicrobial peptide 1
• Gene Symbols: DEFB4A
• Primary or Secondary: Primary
• Content And Storage: -20°C