PeproTech BDNF Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  PeproTech 500-P84-100UG

Product Code. 17883053

Description

AA Sequence of recombinant protein: MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human/Murine/Rat BDNF. Anti-Human/Murine/Rat BDNF-specific antibody was purified by affinity chromatography employing immobilized Human/Murine/Rat BDNF matrix. Sandwich ELISA: To detect Human/Murine/Rat BDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human/Murine/Rat BDNF (500-P84Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human/Murine/Rat BDNF. Western Blot: To detect Human BDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

Specifications

• Antigen: BDNF
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BDNF
• Gene Alias: abrineurin; ANON2; anorexia BDNF; Bdnf; BDNF precursor form; bdnf protein; brain derived neurothrophic factor; brain derived neurotrophic factor; brain-derived neurotrophic factor; BULN2; H-BDNF; neurotrophin; ProBDNF
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12064, 24225, 627
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P21237, P23363, P23560
• Gene Symbols: BDNF
• Immunogen: E.coli-derived Recombinant Human/Murine/Rat BDNF.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody