Phosflow Lyse/Fix Buffer 5X, BD

Product Code.	Quantity	unitSize
15839178	250mL	Each

Product Code. 15839178 Supplier BD Biosciences Supplier No. 558049

Description

For the lysing and fixing of whole blood for use with intracellular flow cytometry

• Can be used for lysing red blood cells and for fixing leukocytes in one step, allowing investigators to rapidly inactivate kinases, phosphatases and/or proteases, so that the in vivo phosphorylation state of the cell can be examined
• Has been reported to preserve the light scattering properties of the cell
• May be used on human and mouse whole blood
• Whole blood lysis has been shown to be as effective as density gradient centrifugation in the preparation of PBMCs for lymphocyte subset analysis
• In many cases, phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g. CD markers); however, not all cell surface markers, however, are compatible with the BD Phosflow Lyse/Fix Buffer

Specifications

• Color: Clear
• Content And Storage: Room Temperature
• Format: Liquid
• Quantity: 250mL
• Validated Application: RUO
• Product Type: Lyse/Fix Buffer

Safety and Handling

Product Identifier

• BD Phosflow Lyse/Fix Buffer

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Acute toxicity Category 4
• Carcinogenicity Category 1B
• Serious eye damage/eye irritation Category 2
• Germ cell mutagenicity Category 2
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1
• Specific target organ toxicity after repeated exposure Category 2
• Specific target organ toxicity Category 2

Hazard Statement

• H241-Heating may cause a fire or explosion.
• H302-Harmful if swallowed.
• H311-Toxic in contact with skin.
• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H331-Toxic if inhaled.
• H335-May cause respiratory irritation.
• H350-May cause cancer.
• H371-May cause damage to organs.
• H373-May cause damage to organs.

Precautionary Statement

• P201-Obtain special instructions before use.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.

Supplemental information

• MIXTURE LIST-Contains : formaldehyde, diethylene glycol, methanol