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BD Caspase-3 Enzyme Set

Product Code. 15898708
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Cross Reactivity:
Human
Unit Size:
Each
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Product Code. 15898708

Brand: BD 556473

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Description

Includes

Recombinant Human Active Caspase-3: 5μg, Ac-DEVD-AFC, Caspase-3 Fluorogenic Substrate: 1.0mg, Ac-DEVD-CHO, Caspase-3 Inhibitor: 1.0mg

Gene that is required for normal apoptosis during development

Caspases are cysteine proteases that play central role in apoptosis. The caspase family was discovered by searching human cDNA libraries for sequences homologous to ced-3, a C. The rate of caspase-3 enzymatic hydrolysis can be measured by release of AFC, from Ac-DEVD-AFC caspase substrate, through emission at 480-520nm (peak at 505nm) upon excitation at 400nm using UV spectrofluorometry

  • Consisting of 12kDa and 17kDa subunit derived from 32kDa pro-enzyme (pro- caspase-3)
  • Cleaves PARP, an enzyme that is involved in DNA repair and genomic maintenance
  • The cleavage site in PARP is C-terminal to Asp-216
  • Cleaves the tetrapeptide between D and AFC, thus releasing the fluorogenic AFC
  • Expressed in E.coli and purified

Enzyme assay

TRUSTED_SUSTAINABILITY

Specifications

For Use With (Application) Enzyme Assay
Name Caspase-3 Enzyme Set
Quantity 20 Tests
Storage Requirements -80°C
Cross Reactivity Human

Research use only

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