Bcl-2 Antibody (BCL2/782), Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18198308	Novus Biologicals NBP2-44783-0.02MG	0.02 mg
18117087	Novus Biologicals NBP2-44783-0.1MG	0.1 mg

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Bcl-2 Monoclonal specifically detects Bcl-2 in Human samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Bcl-2
• Concentration: 0.2mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Autophagy, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Oncogenes, Tumor Suppressors
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
• Gene Symbols: BCL2
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

• Clone: BCL2/782
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P10415
• Gene ID (Entrez): 596
• Immunogen: Recombinant full-length human bcl-2 protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 25.5 kDa

For Research Use Only