Bcl-2 Antibody (100/D5), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 7 publications

Product Code	Brand	Quantity
18788593	Novus Biologicals NBP2-15200-0.1mg	0.1 mg
18172293	Novus Biologicals NBP2-15200-0.2MG	0.2 mg
18491242	Novus Biologicals NBP2-15200-0.02mg	0.02 mg

Description

Bcl-2 Monoclonal specifically detects Bcl-2 in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular).

Specifications

• Antigen: Bcl-2
• Concentration: 0.2mg/mL
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
• Gene Symbols: BCL2
• Isotype: IgG1 κ
• Purification Method: Protein G purified
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 25 kDa

• Clone: 100/D5
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Autophagy, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Tumor Suppressors
• Gene Accession No.: P10415
• Gene ID (Entrez): 596
• Immunogen: The synthetic peptide corresponding to amino acids range from 41-64 (GAAPAPGIFSSQPG-Cys), of human Bcl-2 was used as immunogen to generate the antibody; GenBank no. NP_000648.2 (Pezzella et al, 1990).
• Primary or Secondary: Primary
• Reconstitution: Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
• Test Specificity: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

For Research Use Only