Bax Antibody (2D2), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 1 publication

Brand:  Novus Biologicals NBP2-29468

Product Code. 18189104

Description

Bax Monoclonal specifically detects Bax in Human, Monkey, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Bax
• Classification: Monoclonal
• Concentration: 0.2 mg/ml
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry 0.5-1ug/ml, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
• Gene Alias: apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4
• Host Species: Mouse
• Molecular Weight of Antigen: 21 kDa
• Quantity: 0.1 mg
• Research Discipline: Apoptosis, Cancer, Core ESC Like Genes, Stem Cell Markers, Tumor Suppressors
• Gene ID (Entrez): 581
• Target Species: Human, Monkey, Mouse (Negative), Rat (Negative)
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: 2D2
• Conjugate: Unconjugated
• Gene Accession No.: Q07812
• Gene Symbols: BAX
• Immunogen: A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein. (Uniprot: Q07812)
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 21kDa, identified as the Bax protein. This monoclonal antibody is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

For Research Use Only