Invitrogen™ BAF53A Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA519421

Product Code. 11387393

Description

PA5-19421 targets BAF53A in IF, IHC (F), and WB applications and shows reactivity with Human, mouse, and Rat samples. The PA5-19421 immunogen is synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BAF53A. PA5-19421 detects BAF53A which has a predicted molecular weight of approximately 53 kDa.

BAF53A is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Specifications

• Antigen: BAF53A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Actl6a
• Gene Alias: 2810432C06Rik; 53 kDa BRG1-associated factor A; actin like 6A; actin-like 6; actin-like 6A; actin-like 6B; actin-like protein 6A; actin-related protein 4; actin-related protein Baf53a; Actl6; Actl6a; ACTL6B; AI851094; ARP4; ArpNbeta; ARPN-BETA; BAF complex 53 kDa subunit; BAF53; Baf53a; BAF53B; BRG1/brm-associated factor 53A; BRG1-associated factor 53A; C79802; hArpN alpha; hArpN beta; INO80 complex subunit K; INO80K; MGC5382; MGC5382 Arp4; RGD1307747
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 361925, 56456, 86
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ChIP Assay, Western Blot, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.8 mg/mL
• Formulation: PBS with 1% BSA and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O96019, Q9Z2N8
• Gene Symbols: Actl6a
• Immunogen: Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BAF53A.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG