Cytiva bacteria genomicPrep Mini Spin Kit

Product Code. 10546485

Brand: Cytiva 28904259

Description

Includes

illustra bacteria genomicPrep Mini spin columns prepacked with novel silica membrane; Lyophilized proteinase K powder; Lysis solutions, wash and elution buffers; 2mL Microcentrifuge collection tubes; a full booklet with a detachable, quick reference protocol card

Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria

The illustra Bacteria genomicPrep Mini Spin Kit can be used with a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis etc. The kit produces highly pure and intact genomic DNA with OD_260/280 values of 1.75-1.85. Use different elution volumes to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RT-PCR, and long PCR.

The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.

• Total DNA extraction time under one hour with reduced lysis time
• Can be used to purify 4 to 8μg of genomic DNA from 2 × 10⁹ bacteria cells with input amounts ranging from 1 × 10⁹ to 4 × 10⁹ bacteria cells
• Spin columns prepacked with a silica membrane—provide excellent reproducibility and consistency
• Validated in several downstream applications including RT PCR, long PCR, and restriction digest
• Color-coded caps for ease of use

Specifications

• Description: illustra bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria.
• For Use With (Application): For the rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteriaPurified gDNA is suitable for molecular biology applications including cloning, restriction enzyme digestion, PCR and genotyping
• Includes: 30mg Proteinase K, 3mL Lysis Buffer type 2, 0.6mL Lysis Buffer type 3, 60mL Lysis Buffer type 4, 6mL Wash Buffer type 6, 12mL Elution Buffer type 5, 50 Illustra™ bacteria mini columns, 50 collection tubes
• No. of Reactions: 250 Preps
• Product Type: illustra bacteria genomicPrep Mini Spin Kit
• Purity: 1.7
• Sample Volume: 2 x 10⁹ cells [A₆₀₀ = 2.0]
• Test Time: 40 min [Gram-negaitive Bacteria], 55 min [Gram-positive Bacteria]

Safety and Handling

Signal Word

• Danger

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H335-May cause respiratory irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : proteases