Invitrogen™ BACE1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA519952

Product Code. 12868162

Description

A suggested positive control is human brain tissue lysate. PA5-19952 can be used with blocking peptide PEP-0077. The PA5-19952 immunogen is located within the last 50 amino acids of BACE. Predicted molecular ~ 55kD. In Western blot applications, this antibody has been observed to detect a band at: 65kD (Post-modification: 4 N-linked glycosylation). Predicted species reactivity based on immunogen sequence: Guinea pig: (94%), Rat: (94%), Bovine: (94%).

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimer's disease (AD). In some cases early onset familial AD can be attributed to a swedish mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that have focused on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase stuff. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all sufferers of Alzheimer's disease. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). bACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

Specifications

• Antigen: BACE1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BACE1
• Gene Alias: APP beta-secretase; asp 2; ASP2; Aspartyl protease 2; BACE; BACE 1; BACE1; BACE-1D; BACE-I-432; Beta secretase; Beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; C76936; HSPC104; hypothetical protein LOC403005; KIAA1149; Memapsin; Memapsin2; Memapsin-2; membrane-associated aspartic protease 2; protease; transmembrane aspartic proteinase Asp2; zgc:77409
• Host Species: Rabbit
• Purification Method: Ion-exchange chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 23621, 23821
• Content And Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: P56817, P56818
• Gene Symbols: BACE1
• Immunogen: A peptide corresponding to 17 amino acids at the carboxy terminus of human BACE.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG