Eurofins DiscoverX B-Raf (δ1-415) Protein, Active

Product Code. 17370304

Brand: Eurofins DiscoverX 14530M

Description

Active, N-terminal GST-tagged recombinant human B-Raf residues 416-end, for use in Kinase Assays.

B-RAF plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes.

GenBank NM_004333

Specifications

• Formulation: mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Molecular Weight (g/mol): 67.2 kDa
• Quantity: 250 μg
• Species: Human
• Recombinant: Recombinant
• Protein Tag: N-terminal GST
• Expression System: Recombinant enzyme expressed in Sf21 insect cells
• Protein Form: Truncated
• Purity or Quality Grade: ≥45%
• Protein: B-Raf, active