Azido-PEG3-maleimide Kit

Product Code. 15457169

Brand: Thermo Scientific Alfa Aesar J65984.MB

Description

C₁₅H₂₃N₅O₆

An azido containing reagent with PEG spacer and sulfhydryl reactivity. The compound is not stable upon prolonged storage and is sold as a kit.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
An azido containing reagent with PEG spacer and sulfhydryl reactivity. The compound is not stable upon prolonged storage and is sold as a kit.

Solubility
Difficult to mix.

Notes
Kit contains two components: a) 1.1 eq. of azido-PEG3-amine as an oil; b) 1.0 eq. of maleimide-NHS ester as a solid.

Specifications

Chemical Identifiers

• Molecular Formula: C₁₅H₂₃N₅O₆
• MDL Number: MFCD22380764
• Synonym: N-(11-Azido-3,6,9-trioxaundecyl)-3-(N-maleimidyl)propionamide

• Quantity: 25 mg
• UN Number: UN2735
• Solubility Information: Difficult to mix.
• Formula Weight: 369.37
• Chemical Name or Material: Azido-PEG3-maleimide Kit

Frequently Asked Questions (FAQs)

What storage conditions should I use for the Azido-PEG3-maleimide Kit?

The Azido-PEG3-maleimide Kit should be stored in the dark at -20°C.

What is the protocol for Azide-PEG3-maleimide generation with the Azido-PEG3-maleimide Kit?

We recommend the following protocol for Azide-PEG3-maleimide generation with the Azido-PEG3-maleimide Kit:

1. For the 25 mg kit, add 1 mL of a dry water-miscible solvent (like DMF or DMSO) to the azido-PEG-amine and shake for approximately 30 seconds to ensure mixing. For the 100 mg kit add 2.5 mL of solvent.
2. Place the maleimide-NHS ester under nitrogen or dry atmosphere and slowly add the solution from step 1 with stirring or shaking.
3. Stir or shake the reaction mixture for 30 minutes at room temperature. The progress of the reaction can be followed by Thin Layer Chromatograph.
   Note: For TLC, the solvent is typically methanol:methylene chloride (1:20), run on a silica gel normal phase plate and developed with a potassium permanganate spray. The Rf of the azide-PEG3-maleimide is slightly lower than the Rf for the maleimide-NHS ester. When the reaction is complete, it will be one clean spot on the plate.
4. The reaction product will be ready-to-use azide-PEG3-maleimide.

The azide-PEG3-maleimide is present at ~ 75 mM if 1 mL of solvent is used in step 1. The 25 mg kit produces 0.075 mmol of azide-PEG3-maleimide.

RUO – Research Use Only