Invitrogen™ Axl Monoclonal Antibody (MAXL8DS), Brilliant Violet™ 711, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
30019591	Invitrogen™ 407-1084-82	100 μg
30019384	Invitrogen™ 407-1084-80	25 μg

Description

Axl Monoclonal antibody specifically detects Axl in Mouse samples. It is validated for Flow Cytometry

Description: This MAXL8DS monoclonal antibody recognizes mouse Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3. Within hematopoietic compartment the highest expression of Axl can be observed on dendritic cells and macrophages. Stimulation with TLR ligands has been shown to additionally upregulate this expression. This MAXL8DS antibody will work in flow cytometry on both native and fixed/permeabilized cells. Applications Reported: This MAXL8DS antibody has been reported for use in flow cytometric analysis. Applications Tested: This MAXL8DS antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Brilliant Violet™ 711 (BV711) is a tandem dye that emits at 713 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (<a href='https://www.thermofisher.com/order/catalog/product/SB-4401-42'>Product # SB-4401-42</a>) or Brilliant Stain Buffer™ (<a href='https://www.thermofisher.com/order/catalog/product/00-4409-75'>Product # 00-4409-75</a>) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (<a href='https://www.thermofisher.com/order/catalog/product/00-8222-49'>Product # 00-8222-49</a>) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (<a href='https://www.thermofisher.com/order/catalog/product/00-5333-54'>Product # 00-5333-54</a>) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically. Our internal testing suggests that Brilliant Violet™ 711 (BV711) is not compatible with methanol-based fixation. Excitation: 407 nm; Emission: 713 nm; Laser: Violet Laser. BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Specifications

• Antigen: Axl
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: Adhesion-related kinase; AI323647; ARK; AXL; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; AZF1; JTK11; oncogene AXL; sAXL; soluble AXL; Tyro7; Tyrosine-protein kinase receptor UFO; Ufo; ufo oncogene homolog
• Immunogen: C57BL/6 mouse Axl-human IgG Fc fusion protein.
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: AXL

• Clone: MAXL8DS
• Applications: Flow Cytometry
• Conjugate: Brilliant Violet 711
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: Q00993
• Gene ID (Entrez): 26362
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody