Invitrogen™ AXL Human ELISA Kit

Product Code. 15994204

Brand: Invitrogen™ BMS2286

Description

Sandwich ELISA Kit

The Human AXL ELISA quantitates Hu AXL in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu AXL.

Principle of the method

The Human AXL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

AXL (also known as tyrosine-protein kinase receptor UFO) is a cell surface receptor. The AXL receptor transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6 (GAS6). It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. AXL is an essential epithelial-to-mesenchymal transition-induced regulator of breast cancer metastasis.

Specifications

• Accession Number: P30530
• Assay Range: 31.25-2000 pg/ml
• Assay Sensitivity: 7.457 pg/ml
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 558
• Gene Symbol: AXL
• Interassay CV: 10%
• Intraassay CV: 8.6%
• Kit Contents: Pre-coated 96-well Plate, Standard (2 vials), Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromagen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Serum, 10 μL; Plasma, 10 μL; Cell Lysate,50 μL; Supernatant, 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Human
• Test Time: 1 hr. 20 min.
• Total Assay Time: 3 hr. 30 min.