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Eurofins DiscoverX ATM Protein, Active

Product Code. 17320814
Encompass_Preferred
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Quantity:
10 μg
250 μg
Unit Size:
10µg
250µg
This item is not returnable. View return policy

Product Code. 17320814

Brand: Eurofins DiscoverX 14933

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This item is not returnable. View return policy

Active, Recombinant, human FLAG-tagged ATM full length, for use in Kinase Assays.

ATM is a serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation.

GenBank NM_000051

Specifications

Formulation mg/mL of enzyme in storage buffer.
Molecular Weight (g/mol) 352 kDa
Quantity 10 μg
Species Human
Recombinant Recombinant
Protein Tag FLAG-tag
Expression System Recombinant enzyme expressed in mammalian cells
Protein Form Full-length
Purity or Quality Grade ≥47%
Protein ATM, active
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