Invitrogen™ ATF6 Recombinant Rabbit Monoclonal Antibody (K01_2L46)

Product Code.	Quantity
30827861	100 μL
30828082	50 μL

Product Code. 30827861 Supplier Invitrogen™ Supplier No. MA564000

Description

Rabbit Recombinant Monoclonal Antibody

Immunogen sequence: MGEPAGVAGT MESPFSPGLF HRLDEDWDSA LFAELGYFTD TDELQLEAAN ETYENNFDNL DFDLDLMPWE SDIWDINNQI CTVKDIKAEP QPLSPASSSY SVSSPRSVDS YSSTQHVPEE LDLSSSSQMS PLSLYGENSN SLSSAEPLKE DKPVTGPRNK TENGLTPKKK IQVNSKPSIQ PKPLLLPAAP KTQTNSSVPA KTIIIQTVPT LMPLAKQQPI ISLQPAPTKG QTVLLSQPTV VQLQAPGVLP SAQPVLAVAG GVTQLPNHVV NVVPAPSANS PVNGKLSVTK PVLQSTMRNV GSDIAVLRRQ QRMIKNRESA CQSRKKKKEY MLG Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 82%, Pig - 89%, Cynomolgus monkey - 97%.

ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It Interacts also with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.

Specifications

• Antigen: ATF6
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Recombinant Monoclonal
• Clone: K01_2L46
• Concentration: 0.6 mg/mL
• Conjugate: Unconjugated
• Formulation: 50mM tris glycine with 150mM NaCl, 40% glycerol, 0.05% BSA and 0.01% sodium azide; pH 7.4
• Gene: ATF6
• Gene Accession No.: P18850, Q99941
• Gene Alias: 9130025P16Rik; 9630036G24; AA617266; AA789574; ACHM7; activating transcription factor 6; activating transcription factor 6 alpha; Activating transcription factor 6 beta; ATF 6; Atf6; ATF6 alpha; ATF6A; Atf6alpha; ATF6-alpha; Atf6b; ATF6beta; ATF6-beta; cAMP response element binding protein-related protein; cAMP response element-binding protein-related protein; cAMP responsive element binding protein-like 1; cAMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 beta; cAMP-responsive element-binding protein-like 1; Crebl1; Creb-related protein; CREB-RP; Cyclic AMP-dependent transcription factor ATF-6 alpha; cyclic AMP-dependent transcription factor ATF-6 beta; DKFZp686P2194; ESTM49; FLJ21663; G13; Processed cyclic AMP-dependent transcription factor ATF-6 alpha; Processed cyclic AMP-dependent transcription factor ATF-6 beta; Protein G13
• Gene Symbols: ATF6, ATF6B
• Host Species: Rabbit
• Immunogen: Recombinant protein of human ATF6 (aa 1-333).
• Purification Method: Protein A/G
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1388, 22926
• Target Species: Human
• Content And Storage: Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG