Invitrogen™ ASAH2 Polyclonal Antibody

Sheep Polyclonal Antibody

Brand:  Invitrogen™ PA548033

Product Code. 15578522

Description

In direct ELISAs, approximately 15% cross-reactivity with recombinant human ASAH2 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

Specifications

• Antigen: ASAH2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Asah2
• Gene Alias: acylsphingosine deacylase 2; AI585898; Asah2; BCDase; hCD; HNAC1; LCDase; mitochondrial ceramidase; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; N-acylsphingosine amidohydrolase 2; NCDase; N-CDase; Neutral ceramidase; Neutral ceramidase soluble form; neutral/alkaline ceramidase; non-lysosomal ceramidase
• Host Species: Sheep
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 54447
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Immunoprecipitation, Western Blot
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: Q9JHE3
• Gene Symbols: Asah2
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse ASAH2/N-acylsphingosine Amidohydrolase-2 Thr34-Thr756.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG