PeproTech Artemin Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17824913	PeproTech 500-P245-50UG	50 μg
17875403	PeproTech 500-P245-100UG	100 μg
17885083	PeproTech 500-P245-1MG	1 mg

Description

AA Sequence of recombinant protein: AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Artemin. Anti-Human Artemin-specific antibody was purified by affinity chromatography employing an immobilized Human Artemin matrix. Sandwich ELISA: To detect Human Artemin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Artemin (500-P245Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Artemin. Western Blot: To detect Human Artemin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Artemin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

The protein encoded by this gene is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands which are a group of ligands within the TGF-beta superfamily of signaling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenerative disease. Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: Artemin
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: Q5T4W7
• Gene ID (Entrez): 9048
• Immunogen: E.coli-derived Recombinant Human Artemin.
• Purification Method: Antigen affinity chromatography
• Gene: ARTN

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: Artemin; ARTN; Enovin; EVN; NBN; neublastin; neurotrophic factor
• Gene Symbols: ARTN
• Primary or Secondary: Primary
• Content And Storage: -20°C