Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience™, Invitrogen™

Armenian Hamster Isotype Control

Product Code	Brand	Quantity
15370960	Affymetrix eBioscience 25-4888-81	50 μg
15380960	Affymetrix eBioscience 25-4888-82	100 μg

Description

Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis. Isotype control should be used at the same concentration as experimental antibody. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Specifications

• Antigen: Armenian Hamster IgG
• Concentration: 0.2 mg/mL
• Conjugate: PE-Cyanine7
• Host Species: Armenian Hamster
• Target Species: Not Applicable
• Gene Alias: IgG; Immunoglobulin G; ImmunoglobulinG
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

• Clone: eBio299Arm
• Applications: Control, Flow Cytometry
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Isotype: IgG
• Purification Method: Affinity chromatography

For Research Use Only.