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Invitrogen™ Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 615, eBioscience™

Product Code. 15361110
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25 μg
Unit Size:
25µg
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15361110 25 μg 25µg
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Product Code. 15361110 Supplier Invitrogen™ Supplier No. 42488880

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Description

Armenian Hamster Monoclonal Isotype Control

The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: This eBio299Arm antibody has been reported for use in immunohistochemical staining and immunocytochemistry. Applications Tested: This eBio299Arm antibody has been tested by immunocytochemistry and/or immunohistochemistry. Use isotype control at the same concentration as experimental antibody. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength. (for example Excitation 560/55, 585LP, Emission 645/75) A standard Alexa Fluor 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 μm post-manufacturing filtered.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Armenian Hamster IgG
Applications Control, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry
Classification Monoclonal
Clone eBio299Arm
Concentration 0.2 mg/mL
Conjugate eFluor 615
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Alias IgG; Immunoglobulin G; ImmunoglobulinG
Host Species Armenian Hamster
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Isotype Control (Primary)
Target Species Not Applicable
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Isotype Control
Form Liquid
Isotype IgG
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