Invitrogen™ ARIH1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA567280

Product Code. 15959944

Description

Immunogen sequence: NHMVCRNQNCK AEFCWVCLGP WEPHGSAWYN CNRYNEDDAK AARDAQERSR AALQRYLFYC NRYMNHMQSL RFEHKLYAQV KQKMEEMQQH N Highest antigen sequence identity to the following orthologs - mouse 100%, rat 100%.

E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:15236971, PubMed:21532592, PubMed:24076655, PubMed:27565346, PubMed:23707686). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets (PubMed:27565346). The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2 (PubMed:27565346). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes (PubMed:24076655, PubMed:27565346). Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear (PubMed:25624349). According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed:25624349). According to another report EIF4E2 ubiquitination leads to its subsequent degradation (PubMed:14623119). Acts as the ligase involved in ISGylation of EIF4E2 (PubMed:17289916). In vitro, controls the degradation of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex member SUN2 and may therefore have a role in the formation and localization of the LINC complex, and as a consequence, nuclear subcellular localization and nuclear morphology (PubMed:29689197). [UniProt].

Specifications

• Antigen: ARIH1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ARIH1
• Gene Alias: ARI; ARI-1; ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1; ariadne RBR E3 ubiquitin protein ligase 1; ariadne, Drosophila, homolog of; ARIH1; DKFZp686O13120; E3 ubiquitin-protein ligase ARIH1; FLJ20329; FLJ93118; H7-AP2; HARI; HHARI; HUSSY-27; Monocyte protein 6; MOP6; MOP-6; Protein ariadne-1 homolog; ubcH7-binding protein; UBCH7BP; ubcM4-interacting protein; Ubiquitin-conjugating enzyme E2-binding protein 1
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 25820
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry
• Concentration: 0.05 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q9Y4X5
• Gene Symbols: ARIH1
• Immunogen: Recombinant Human ARIH1. Recombinant protein control fragment (Product #RP-107917).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG