Invitrogen™ Arginase 1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA529645

Product Code. 13433857

Description

Recommended positive controls: Mouse liver, rat liver. Predicted reactivity: Mouse (85%), Rat (83%), Pig (90%), Rabbit (90%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

Specifications

• Antigen: Arginase 1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ARG1
• Gene Alias: AI; A-I; AI type I arginase; AI256583; Arg1; Arg-1; Arginase; arginase 1; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase1; arginase-1; HGNC:663; Liver Arginase; Liver-type arginase; PGIF; similar to arginase, type I; Type 1 Arginase; type I arginase
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11846, 29221, 383
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.41 mg/mL
• Formulation: PBS with 20% glycerol, 1% BSA and 0.025% ProClin 300; pH 7
• Gene Accession No.: P05089, P07824, Q61176
• Gene Symbols: ARG1
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Arginase 1. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG