Invitrogen™ Arginase-1 Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
16312004	Invitrogen™ BMS2216	96 Tests
16322004	Invitrogen™ BMS2216TEN	96 x 10 Tests

Description

The Arginase-1 Human ELISA Kit quantitates Hu Renin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Arginase-1.

Principle of the method

The Human Arginase-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Arginase-1 is a 35 kDa enzyme that that helps eliminate nitrogen by converting L-arginine to urea and L-ornithine which further converts to polyamine. These polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. Expression is found in the liver, neutrophils, as well as myeloid derived suppressor cells (MDSC) and neural stem cells. Expression in macrophages has not been determined in humans, while in mice expression in M2 macrophages is well established. Arginase-1 may be expressed in the myeloid population in breast cancer tumors and is typically found in the majority of hepatocellular carcinomas.

Specifications

• Accession Number: P05089
• Assay Sensitivity: 20.81 pg/ml
• Product Type: ELISA Kit
• Target Species: Human
• Gene ID (Entrez): 383
• Interassay CV: 8.5%
• Kit Contents: Pre-coated 96-well Plate, Standard (2 vials), Sample Diluent, Assay Buffer Concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 78.1–5000 pg/ml
• Conjugate: Biotin
• Sample Type: Cell Lysates, Plasma, Serum
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: ARG1
• Intraassay CV: 6.6%
• Label or Dye: HRP
• Sample Volume: Serum, 50 μL; Plasma, 50 μL; Cell Lysate,50 μL
• Target: Human
• Total Assay Time: 3 hr. 30 min.