Invitrogen™ Aquaporin 0 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA536535

Product Code. 15583911

Description

This antibody detects endogenous protein at a molecular weight of 28 kDa. Purity is >95% by SDS-PAGE.

AQP0 is the most abundant endogenous protein in the plasma membrane of lens fiber cells where it functions not only as a water pore, but it is also involved in fiber-fiber adhesion and is crucial for fiber cell structure and organization. AQP0 contains an additional pore constriction, not seen in any other aquaporin structures, which may be responsible for pore gating. The closed AQP0 pore holds just three water molecules, which are spaced too far apart to form hydrogen bonds with each other. The C-terminal domain of AQP0 undergoes extensive post-translational modification, including many truncations, during lens aging due to the actions of m-calpain, proteases or non-enzymatic mechanisms. These truncation sites may be involved in the development of cataracts.

Specifications

• Antigen: Aquaporin 0
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MIP
• Gene Alias: AQP0; aquaporin 0; Aquaporin0; aquaporin-0; Cat; CTRCT15; dominant cataract; Hfi; hydropic fibers; lens fiber major intrinsic protein; lens opacity; LIM1; Lop; major intrinsic protein of eye lens fiber; major intrinsic protein of lens fiber; MIP; MIP26; MP26; Palm; shrivelled; Svl
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17339, 25480, 4284
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: P09011, P30301, P51180
• Gene Symbols: MIP
• Immunogen: Synthetic peptide corresponding to amino acids 110-160 of human AQP0.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG