PeproTech APRIL Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17855763	PeproTech 500-P192-50UG	50 μg
17825243	PeproTech 500-P192-100UG	100 μg
17865583	PeproTech 500-P192-1MG	1 mg

Description

AA Sequence of recombinant protein: AVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQAQGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF RCIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPHGTF LGFVKL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human APRIL. Anti-Human APRIL-specific antibody was purified by affinity chromatography employing an immobilized Human APRIL matrix. Sandwich ELISA: To detect Human APRIL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human APRIL (500-P192Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human APRIL. Western Blot: To detect Human APRIL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human APRIL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for A PRoliferation-Inducing Ligand), TALL2 (for TNF and ApoL-related Leukocyte-expressed Ligand 2), and TRDL 1a (for TNF related death ligand 1a) in human and mouse. Two receptors for APRIL were recently identified and designated TACI and BCMA. APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kB, and induces cell death. APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer.

Specifications

• Antigen: APRIL
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: O75888
• Gene ID (Entrez): 8741
• Immunogen: Insect cells-derived Recombinant Human APRIL.
• Purification Method: Antigen affinity chromatography
• Gene: TNFSF13

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: 2310026N09Rik; A proliferation-inducing ligand; APRIL; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; Tnfsf13; TNLG7B; Trdl1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand 7B; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; UNQ383/PRO715; ZTNF2
• Gene Symbols: TNFSF13
• Primary or Secondary: Primary
• Content And Storage: -20°C