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PeproTech APRIL Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
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Product Code. 17855063
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2 x 500 μg
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500 μg
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500µg
50µg
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17855063 25 μg 25µg
17855353 50 μg 50µg
17895743 2 x 500 μg 1mg
30054702 500 μg 500µg
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Product Code. 17855063 Supplier PeproTech Supplier No. 500P192BT25UG

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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: AVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQAQGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF RCIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPHGTF LGFVKL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human APRIL. Anti-Human APRIL-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hAPRIL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human APRIL (500-P192) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hAPRIL. Western Blot: To detect Human APRIL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human APRIL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P192BT-1MG will be provided as 2 x 500 μg

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for A PRoliferation-Inducing Ligand), TALL2 (for TNF and ApoL-related Leukocyte-expressed Ligand 2), and TRDL 1a (for TNF related death ligand 1a) in human and mouse. Two receptors for APRIL were recently identified and designated TACI and BCMA. APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kB, and induces cell death. APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer.
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Specifications

Antigen APRIL
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Biotin
Formulation PBS with no preservative
Gene TNFSF13
Gene Accession No. O75888
Gene Alias 2310026N09Rik; A proliferation-inducing ligand; APRIL; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; Tnfsf13; TNLG7B; Trdl1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand 7B; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; UNQ383/PRO715; ZTNF2
Gene Symbols TNFSF13
Host Species Rabbit
Immunogen Insect cells-derived Recombinant Human APRIL.
Purification Method Antigen affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 8741
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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