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Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG
Shop All Applied Biosystems ProductsDescription
- Contains AmpliTaq Gold™ DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq™ DNA polymerase
- Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems™ real-time PCR instruments
- Buffer enhancements guarantee performance and reliability in all applications, even with G/C-rich sequences
- Contains dUTP in the dNTP mix to protect against carry-over contamination, when used with AmpErase™ UNG
- Rapid assay development guidelines are provided to minimize optimization time
Order Info
Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice.
Specifications
Specifications
| Content And Storage | Supplied at 2X concentration. The mix contains AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 and optimized buffer components. This pack contains 10 X 5 mL tubes, sufficient for 2000 reactions at the 50 μL total volume. Store at 2-8°C Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
| Detection Method | Primer-probe |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Reaction Speed | Standard |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Passive Reference Dye | ROX (Pre-mixed) |
| Product Line | TaqMan |
| Product Type | Universal PCR Master Mix |
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Frequently Asked Questions (FAQs)
We recommend using either the TaqPath ProAmp Master Mix or the TaqMan Genotyping Master Mix. The TaqMan Genotyping Master Mix has the advantage of proven performance with up to 3 days of pre- and post-PCR stability, allowing you to set up plates ahead of time or read the plates later (see the data here, https://tools.thermofisher.com/content/sfs/brochures/cms_039236.pdf) while the TaqPath ProAmp Master Mix can handle samples that may have inhibitors present.
For Research Use Only. Not for use in diagnostic procedures.
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