Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

For use with reverse transcriptase-PCR (RT-PCR)

Overview
Brand: Applied Biosystems™

Manufacturer Part Number: N8080234

TQMN,REV TRANSCRIPTION RGNTS/reactions set up at 10 ┬ÁL each

UNSPSC: 41106313

Code: 50

Additional Details:
Additional Details: Weight: 0.07000kg



Product Code. 10117254

Quantity Price
1 £ 316.0 / Each
Estimated Shipment date
from Supplier 13-12-2016
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

For Use With (Application) RT-PCR (Endpoint), Real-Time PCR
Format SuperMix or Master Mix, Separate Components
Includes 1 × 100μL of MultiScribe Reverse Transcriptase (50U/μL); 2 × 200μL of RNase Inhibitor (20U/μL); 1 × 1mL of dNTP Mixture (2.5mM each dNTP); 1 × 100μL of Oligo d(T)16 (50μM); 1 × 100μL of Random Hexamers (50μM); 1 × 1.5mL of 10X RT Buffer; 1 × 1.5mL of MgCl2 Solution
Quantity 200 reactions
Sample Size 10μL
Sample Type RNA
Storage Requirements Store between -15deg. to -25deg.C.
Temperature (Metric) Operating 42deg.C
Recombinant Yes

Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

  • Contain MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
  • Random hexamers and oligo (dT)16 included for flexibility
  • Individual components provide versatility in assay set-up
    •Compatible with one-step or two-step RT-PCR reactions
  • Optimal reaction temperature: 42°C
  • Final product size: 5kb or less

Using TaqMan Reverse Transcription Reagents TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems. Built-in normalization The TaqMan reverse transcription reagents contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Applications:

PCR & Real-Time PCR, Reverse Transcription