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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

For use with reverse transcriptase-PCR (RT-PCR)

Brand:  Applied Biosystems™ N8080234

352.80 GBP valid until 2021-10-30
Use promo code "14806" to get your promotional price.



Code : 50

Additional Details : Weight : 0.07000kg

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Product Code. 10117254

  • £401.00 / Each

In stock, ready to ship: 18

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

  • Contain MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
  • Random hexamers and oligo (dT)16 included for flexibility
  • Individual components provide versatility in assay set-up
  • Compatible with one-step or two-step RT-PCR reactions
  • Optimal reaction temperature: 42°C
  • Final product size: 5kb or less

Using TaqMan Reverse Transcription Reagents TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.

Built-in normalization The TaqMan reverse transcription reagents contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Applications:
PCR & Real-Time PCR, Reverse Transcription

Specifications

Specifications

42° C
MultiScribe™
Dry Ice
cDNA (First-Strand)
Tube
RT-PCR
Reverse Transcription Reagent
5 kb or less
200 reactions
RNA
Primer-Probe
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
200 Reactions
TaqMan™
SuperMix or Master Mix, Separate Components
200 Reactions
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SDS
Documents
Certificates
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Special Offers

Special Offers

  • Big discounts are available on a range of popular products including chemicals, lab essentials, equipment, consumables, life science and safety products.
    : 14806

    2021-10-31

    *Discount will apply to qualifying orders received by Fisher Scientific between 01/09/2021 and 31/10/2021. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

    Terms & Conditions

For Research Use Only. Not for use in diagnostic procedures.