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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

Brand:  Applied Biosystems™ 4304134

2860.14 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 50

Additional Details : Weight : 0.50000kg

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Product Code. 10279624

  • £4290.00 / 1 set
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and polymerase chain reaction amplification of a specific target RNA from either total RNA of mRNA. The reagents in this kit facilitate two-step RT-PCR.

Two-step RT-PCR is performed in two separate reactions, useful when detecting multiple transcripts from a single cDNA reaction, or when storing a portion of the cDNA.

Advantages of TaqMan Reverse Transcription Reagents
• Contains MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)16 included for flexibility
• Individual components provide versatility in assay setup
• Compatible with two-step RT-PCR reactions

Using TaqMan Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.

Specifications

Specifications

Includes 10 units of Cat. No. N8080234. Each unit contains:

• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)
• RNase Inhibitor, 100 μL (20 U/μL)
• dNTP Mixture, 1 mL (2.5 mM each dNTP)
• Oligo (dT)16, 100 μL (50 μM)
• Random Hexamers, 100 μL (50 μM)
• 10X RT Buffer, 1.5 mL
• MgCl2 Solution, 1.5 mL

Store at –15 to –25°C.

Reverse Transcription
MultiScribe
Dry Ice
50 U/μL
2,000 Reactions
Reverse Transcription
RNA
Kit
42°C
Yes
Real Time PCR (qPCR)
First-Strand cDNA
Separate components
Up to 5 kb
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For Research Use Only. Not for use in diagnostic procedures.