Applied Biosystems™ TaqMan™ PreAmp Master Mix
TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample.
Brand: Applied Biosystems™ 4488593
Code : NEW
Additional Details : Weight : 0.00500kg
DescriptionTaqMan™ PreAmp Master Mix lets you: • Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE) • Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan™ Gene Expression assays • Analyze up to 100 gene expression targets with minimal hands-on time Preserves Equilibrium of Targets Without Preamplification Bias: In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan™ PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA. Easy Workflow: With TaqMan™ PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan™ assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan™ PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp™ 9700 thermal cycler. For Research Use Only. Not for use in diagnostic procedures.
Guaranteed minimum shelf life is 60 days.
|Detection Method: Primer-Probe Detection, PCR Method: qPCR|
|Real time PCR (qPCR), real time PCR-based gene expression profiling|
|Contains 2X TaqMan™ PreAmp Master Mix (1mL).|
|Passive Reference Dye: No ROX|
|7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, QuantStudio™ 12K Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7|
|Store at 2° to 8°C.|