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Applied Biosystems™ SYBR™ Select Master Mix
Cost-effective solution for real-time PCR applications.
Brand: Applied Biosystems™ 4472913
Code : VX
Additional Details : Weight : 0.00450kg
Description
- Specific - minimize primer-dimer and non-specific amplification
- Reproducible and sensitive - consistent amplification across a wide dynamic range
- Bright - contains SYBR™ GreenER™ dye for maximum brightness
- Carry-over contamination control - contains heat-labile UDG
- Can be used in either standard or fast cycling mode
Formulated for Maximum Specificity:
- SYBR™ Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix
- The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity
Obtain Reproducible Results Across a Wide Dynamic Range:
- SYBR™ Select Master Mix is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA
Contains SYBR™ GreenER™ Dye and Heat-labile UDG:
- SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye resulting in brighter signals
- Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control
Instrument Compatibility:
- SYBR™ Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments except the 7900HT real-time PCR system
- It is also compatible with the Bio-Rad IQ™5, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
Order Info
Shipping Conditions: Wet Ice
Specifications
Purity or Quality Grade: UP (Ultra Pure) | |
The 2X mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 x 1mL tube, sufficient for 100, 20μL reactions (10μL Master Mix per reaction). | |
Store at 2° to 8°C. | |
Detection Method: SYBR; PCR Method: qPCR | |
DNA (Genomic), cDNA |
Real time PCR (qPCR), real time PCR-based gene expression profiling | |
1 x 50mL | |
Primer-Probe Compatability: Unlabeled Oligos | |
7500 Fast System, 7500 System, QuantStudio™ 12K Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System | |
Fast, Standard |
For Research Use Only. Not for use in diagnostic procedures.