Applied Biosystems™ SYBR™ Select Master Mix

Cost-effective solution for real-time PCR applications.

Brand:  Applied Biosystems™ 4472913

Product Code. 13286519

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.

Description

• Specific - minimize primer-dimer and non-specific amplification
• Reproducible and sensitive - consistent amplification across a wide dynamic range
• Bright - contains SYBR™ GreenER™ dye for maximum brightness
• Carry-over contamination control - contains heat-labile UDG
• Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity:

• SYBR™ Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix
• The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity

Obtain Reproducible Results Across a Wide Dynamic Range:

• SYBR™ Select Master Mix is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA

Contains SYBR™ GreenER™ Dye and Heat-labile UDG:

• SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility:

• SYBR™ Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments except the 7900HT real-time PCR system
• It is also compatible with the Bio-Rad IQ™5, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems

Order Info

Shipping Conditions: Wet Ice

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Specifications

• Product Type: Purity or Quality Grade: UP (Ultra Pure)
• Includes: The 2X mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 x 1mL tube, sufficient for 100, 20μL reactions (10μL Master Mix per reaction).
• Storage Requirements: Store at 2° to 8°C.
• Detection Method: Detection Method: SYBR; PCR Method: qPCR
• Sample Type: DNA (Genomic), cDNA

• For Use With (Application): Real time PCR (qPCR), real time PCR-based gene expression profiling
• Quantity: 1 x 50mL
• Compatibility: Primer-Probe Compatability: Unlabeled Oligos
• For Use With (Equipment): 7500 Fast System, 7500 System, QuantStudio™ 12K Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
• Speed: Fast, Standard