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Applied Biosystems™ SYBR™ Green PCR Master Mix

Everything needed for SYBR™ Green dye–based PCR amplification and detection in a convenient, single-tube format.

Brand:  Applied Biosystems™ 4364346

£628.00 valid until 31/05/2017
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UNSPSC : 41106300

Code : 50

Additional Details : Weight : 0.06000kg

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Product Code. 10177204

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications

Description

  • Premixed components stored at 2° to 8°C significantly reduce assay setup time
  • SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
  • AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
  • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
  • Proprietary buffer enhancements ensure performance and reliability

Maximum Flexibility and Convenience:
  • Applied Biosystems™ SYBR™ Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
  • SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
  • The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
  • Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
  • This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
  • SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
Description & Specifications

Specifications

SYBR™ Green PCR Master Mix
The 5 x 5mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 1000 reactions based on a 50μL reaction volume.
5 x 5mL
SYBR™ Green PCR Master Mix, 5 x 5mL, SYBR Detection Method, qPCR PCR Method, AmpliTaq Gold™ DNA Polymerase, Standard Reaction Speed, cDNA, DNA (Genomic) Sample Type (Specific), Used for Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling
Detection Method: SYBR; PCR Method: qPCR
DNA (Genomic), cDNA
Real time PCR (qPCR), real time PCR-based gene expression profiling
1000 rxn(s)
Store at 2° to 8°C.
Primer-Probe Compatability: Unlabeled Oligos
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Standard
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