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Applied Biosystems™ SYBR™ Green PCR Master Mix

Everything needed for SYBR™ Green dye–based PCR amplification and detection in a convenient, single-tube format.

Brand:  Applied Biosystems™ 4364344

Code : 50

Additional Details : Weight : 0.02000kg

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Product Code. 10418454

  • £530.00 / 1 set


EU Stock 224
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For Research Use Only. All usage must comply with product instructions.
 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

  • Premixed components stored at 2° to 8°C significantly reduce assay setup time
  • SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
  • AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
  • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
  • Proprietary buffer enhancements ensure performance and reliability

Maximum Flexibility and Convenience:
  • Applied Biosystems™ SYBR™ Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
  • SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
  • The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
  • Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
  • This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
  • SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
Specifications

Specifications

Real time PCR (qPCR), real time PCR-based gene expression profiling
2 x 5mL
Primer-Probe Compatability: Unlabeled Oligos
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Standard
The 2 x 5mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 400 reactions based on a 50μL reaction volume.
Store at 2° to 8°C.
Detection Method: SYBR; PCR Method: qPCR
DNA (Genomic), cDNA
Special Offers

Special Offers

  • This issue of Life in the Lab, “Illuminating the immune system”
    : 12863

    2017-12-31

    *Discount will apply to qualifying orders received by Fisher Scientific between 19/10/2017 and 31/01/2018. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

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