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Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Brand:  Applied Biosystems™ 4364344

493.35 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 50

Additional Details : Weight : 0.02000kg

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Product Code. 10418454

  • £740.00 / 1 set
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

  • SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
  • AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
  • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
  • Proprietary buffer enhancements ensure performance and reliability
  • Premixed components stored at 2° to 8°C significantly reduce assay setup time

Maximum Flexibility and Convenience:

  • Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
  • SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
  • The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
  • Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
  • This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
  • SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
  • Store at -20°C
Specifications

Specifications

The 2 x 5 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 400 reactions based on a 50 μl reaction volume.

Store at -20°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Tube
qPCR
Standard
SYBR Green
SYBR™
2 x 5 mL
Gene Expression
400 Reactions
2 x 5 mL
SYBR
High
AmpliTaq Gold DNA Polymerase
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
ROX (Pre-mixed)
Real Time PCR SYBR Master Mix
400 Reactions of 50 μL
2X
DNA (Genomic), cDNA
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For Research Use Only. Not for use in diagnostic procedures.