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Applied Biosystems™ MeltDoctor™ HRM Reagent Kit
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Includes
AmpliTaq Gold™ 360 DNA Polymerase, AmpliTaq Gold 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, MeltDoctor HRM Dye (20X)
High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.
High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.
This kit includes:
- AmpliTaq Gold™ 360 DNA Polymerase which, when combined with AmpliTaq Gold™ 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold™ 360 DNA Polymerase delivers 360° coverage for a full range of targets
- GeneAmp™ dNTP Blend
- MeltDoctorHRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes
Gene Expression Analysis & Genotyping, Genetic Variation Analysis Using Real-Time PCR, Genotyping & Genomic Profiling, High Resolution Melting (HRM) Analysis, Methylation Analysis, Methylation-Sensitive HRM Analysis, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research, Real Time PCR (qPCR)
Order Info
Guaranteed minimum shelf life is 60 days.
Specifications
Specifications
| Content And Storage | This kit includes: AmpliTaq Gold™ 360 DNA Polymerase (2 x 50 μL, each at 5 U/μL), AmpliTaq Gold™ 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, and MeltDoctor™ HRM Dye (20x). The kit provides sufficient materials for 250 20-μL reactions when using 2 U AmpliTaq Gold 360 DNA Polymerase per reaction. Store at -15° to -25° Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
| Detection Method | HRM |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polymerase | AmpliTaq Gold 360 DNA Polymerase |
| Technique | HRM (High Resolution Melting) Analysis, Mutation Scanning |
| For Use With (Equipment) | 7500 Fast System, 7900HT Fast System |
| Genotyping Target | SNPs (Unknown or Numerous), Epigenetic Targets |
| Label or Dye | High Resolution Melting (HRM), SYTO 9 |
| Product Line | MeltDoctor |
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For Research Use Only. Not for use in diagnostic procedures.
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