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Applied Biosystems™ MeltDoctor™ HRM Master Mix

Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.

Brand:  Applied Biosystems™ 4415450

Code : 50

Additional Details : Weight : 0.50000kg

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Product Code. 10209164

  • £3320.00 / 50mL


EU Stock 3
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For Research Use Only. All usage must comply with product instructions.
 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Requires : Template and primers

Description

Description

  • Low background fluorescence
  • High brightness in the presence of double-stranded DNA
  • Minimal temperature shift of DNA melting due to dye binding
  • Thermal stability to tolerate PCR cycling conditions
  • No inhibition of polymerase activity, resulting in high PCR efficiency
  • A dNTP blend including dUTP, which minimizes carryover contamination by allowing amplicon degradation by uracil DNA glycosylase (UDG) in subsequent PCR reactions

Perform Mutation Scanning:
  • HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis
  • As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High- Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE)

Advantages of HRM for mutation scanning
  • Low reagent consumption, with little waste: HRM requires only a 20μL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels
  • Simple, fast workflow: no additional instrumentation is required after PCR amplification;A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis
  • Fast optimization: unlike DHPLC, thermal optimization is not required
  • Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction
    Quality Components
    • Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results
    • AmpliTaq Gold™ 360 DNA Polymerase, a highly purified DNA polymerase that provides hot-start performance, minimizing non-specific product formation and enabling reactions to be set up at room temperature
    • MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

    Order Info

    Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice

Specifications

Specifications

Fluorescent
7500 Fast System, 7900HT Fast System, QuantStudio™ 12K Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, StepOne™ System, StepOnePlus™ System
DNA (Genomic), cDNA
5000 Reactions
7500 Fast System, 7900HT Fast System
10 x 5mL
Dye or Label: HRM (High Resolution Melting) Dye, SYTO™ 9
AmpliTaq Gold™ 360 DNA Polymerase
Store at -15° to -25°C.
Genotyping Target: Epigenetic targets, SNPs (Unknown or Numerous)
2X mixture of AmpliTaq Gold 360 DNA Polymerase, MeltDoctor HRM Dye, a dNTP blend including dUTP, Magnesium salts and other buffer components
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