MeltDoctor™ HRM Master Mix

Formulated for superior HRM performance across a wide range of genomic targets

Brand: ABI Applied Biosystems

Manufacturer Part Number: 4415440


UNSPSC: 41106302

Code: 50

Additional Details:
Additional Details: Weight: 0.10500kg

Requires: Template and primers

Product Code. 10351235

Quantity Price
1 £ 415.0 / Each
EU Stock 17
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


For Use With (Application) 7500 Fast System, 7900HT Fast System, QuantStudio™ 12K Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
Includes 2X mixture of AmpliTaq Gold 360 DNA Polymerase, MeltDoctor HRM Dye, a dNTP blend including dUTP, Magnesium salts and other buffer components
Quantity 5mL

MeltDoctor HRM Master Mix contains all components (except template and primers) for High Resolution Melting analysis, a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. No additional mixing required prior to use.

  • Low background fluorescence
  • High brightness in the presence of double-stranded DNA
  • Minimal temperature shift of DNA melting due to dye binding
  • Thermal stability to tolerate PCR cycling conditions
  • No inhibition of polymerase activity, resulting in high PCR efficiency
  • A dNTP blend including dUTP, which minimizes carryover contamination by allowing amplicon degradation by uracil DNA glycosylase (UDG) in subsequent PCR reactions

  • Perform Mutation Scanning HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis. As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High-Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE). Specifically, the advantages of HRM for mutation scanning include: •Low reagent consumption, with little waste: HRM requires only a 20μL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels
  • Simple, fast workflow: no additional instrumentation is required after PCR amplification. A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis
  • Fast optimization: unlike DHPLC, thermal optimization is not required
  • Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction Quality Components
  • Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results
  • AmpliTaq Gold™ 360 DNA Polymerase, a highly purified DNA polymerase that provides hot-start performance, minimizing nonspecific product formation and enabling reactions to be set up at room temperature
  • MeltDoctor HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

Gene Expression Analysis & Genotyping, Genetic Variation Analysis Using Real-Time PCR, Genotyping & Genomic Profiling, High Resolution Melting (HRM) Analysis, Methylation Analysis, Methylation-Sensitive HRM Analysis, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research, Real Time PCR (qPCR)

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