Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

Product Code	Brand	Includes	Quantity
10400745	Applied Biosystems™ 4368814	1 × 1mL of 10X RT Buffer; 1 × 1mL of 10X RT Random Primers; 1 × 0.2mL of 25X dNTP Mix (100mM); 1 × 0.2mL of MultiScribe Reverse Transcriptase (50U/μL)	200 reactions
10186954	Applied Biosystems™ 4368813	2 × 1mL of 10X RT Buffer; 2 × 1mL of 10X RT Random Primers; 1 × 1mL of 25X dNTP Mix (100mM); 1 × 1mL of MultiScribe Reverse Transcriptase (50U/μL)	1000 reactions

Description

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in single 20μL reaction. Reactions can be scaled up to 100μL to generate 10μg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• Ten-fold greater dynamic range than other kits
• Optimal reaction temperature: 37°C
• Final product size: 7kb or less

• Extensively tested with a variety of templates
• Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

• Format: Separate Components
• For Use With (Application): cDNA Reverse Transciption, RT-PCR (Endpoint), Real-Time PCR
• Sample Type: RNA
• Temperature (Metric) Operating: 37°C

For Research Use Only. Not for use in diagnostic procedures.