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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit
Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA
£279.00 - £1156.00
Specifications
For Use With (Application) | Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR) |
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Product Code | Brand | Includes | Quantity | Price | Quantity & Availability | ||||
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Product Code | Brand | Includes | Quantity | Price | Quantity & Availability | ||||
10400745
|
Applied Biosystems™
4368814 |
1 × 1mL of 10X RT Buffer; 1 × 1mL of 10X RT Random Primers; 1 × 0.2mL of 25X dNTP Mix (100mM); 1 × 0.2mL of MultiScribe Reverse Transcriptase (50U/μL) | 200 reactions |
£279.00
200 reactions |
In stock, ready to ship: 325 | Please sign in to purchase this item. Need a web account? Register with us today! | |||
10186954
|
Applied Biosystems™
4368813 |
2 × 1mL of 10X RT Buffer; 2 × 1mL of 10X RT Random Primers; 1 × 1mL of 25X dNTP Mix (100mM); 1 × 1mL of MultiScribe Reverse Transcriptase (50U/μL) | 1000 reactions |
£1156.00
1000 reactions |
In stock, ready to ship: 127 | Please sign in to purchase this item. Need a web account? Register with us today! | |||
Description
The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in single 20μL reaction. Reactions can be scaled up to 100μL to generate 10μg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.
- Linear target amplification for real-time PCR
- Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
- Ten-fold greater dynamic range than other kits
- Optimal reaction temperature: 37°C
- Final product size: 7kb or less
- Extensively tested with a variety of templates
- Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA
PCR & Real-Time PCR, Reverse Transcription
Order Info
Shipping Condition: Dry ice
Specifications
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR) |
For Research Use Only. Not for use in diagnostic procedures.