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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

£264.00 - £1080.00

Specifications

Format Separate Components
For Use With (Application) cDNA Reverse Transciption, RT-PCR (Endpoint), Real-Time PCR
Sample Type RNA
Temperature (Metric) Operating 37°C
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Products 2
Product Code Brand Includes Quantity Price Quantity & Availability  
Product Code Brand Includes Quantity Price Quantity & Availability  
10400745 View Documents Applied Biosystems™
4368814
1 × 1mL of 10X RT Buffer; 1 × 1mL of 10X RT Random Primers; 1 × 0.2mL of 25X dNTP Mix (100mM); 1 × 0.2mL of MultiScribe Reverse Transcriptase (50U/μL) 200 reactions £264.00
200 reactions View alternative products
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10186954 View Documents Applied Biosystems™
4368813
2 × 1mL of 10X RT Buffer; 2 × 1mL of 10X RT Random Primers; 1 × 1mL of 25X dNTP Mix (100mM); 1 × 1mL of MultiScribe Reverse Transcriptase (50U/μL) 1000 reactions £1080.00
1000 reactions View alternative products
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EU Stock 62 Please to purchase this item. Need a web account? Register with us today!
Description

Description

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in single 20μL reaction. Reactions can be scaled up to 100μL to generate 10μg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

  • Linear target amplification for real-time PCR
  • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
  • Ten-fold greater dynamic range than other kits
  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

  • Extensively tested with a variety of templates
  • Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

Specifications

Separate Components
cDNA Reverse Transciption, RT-PCR (Endpoint), Real-Time PCR
RNA
37°C

For Research Use Only. Not for use in diagnostic procedures.