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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

£313.00 - £1296.00

Specifications

Detection Method Primer-Probe
Final Product Type cDNA (First-Strand)
GC-Rich PCR Performance High
PCR Method RT-PCR, RT-qPCR
Reagent Type Reverse Transcription
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10400745
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Applied Biosystems™
4368814
200 Reactions
£313.00
200 reactions
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10186954
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Applied Biosystems™
4368813
1000 Reactions
£1296.00
1000 reactions
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Description

Description

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in single 20μL reaction. Reactions can be scaled up to 100μL to generate 10μg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

  • Linear target amplification for real-time PCR
  • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
  • Ten-fold greater dynamic range than other kits
  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

  • Extensively tested with a variety of templates
  • Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

Specifications

Primer-Probe
High
Reverse Transcription
cDNA (First-Strand)
RT-PCR, RT-qPCR
RNA
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.