Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

Brand:  Applied Biosystems™ 4368813

Product Code. 10186954

Description

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in single 20μL reaction. Reactions can be scaled up to 100μL to generate 10μg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• Ten-fold greater dynamic range than other kits
• Optimal reaction temperature: 37°C
• Final product size: 7kb or less

• Extensively tested with a variety of templates
• Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA

PCR & Real-Time PCR, Reverse Transcription

Specifications

• Content And Storage: • 2 x 1 mL 10X RT Buffer
  • 2 x 1 mL 10X RT Random Primers
  • 1 x 1 mL 25X dNTP Mix (100 mM)
  • 1 x 1 mL MultiScribe Reverse Transcriptase (50 U/μL)
  
  Store between -15°C and -25°C.
• Detection Method: Primer-Probe
• Final Product Type: cDNA (First-Strand)
• GC-Rich PCR Performance: High
• PCR Method: RT-PCR, RT-qPCR
• Reaction Speed: Standard
• Sample Type: RNA
• Starting Material: RNA
• Volume: 100 μL, 20 μL
• Product Type: High-Capacity cDNA Reverse Transcription Kit
• Sufficient For: 1000 Reactions

• Concentration: 50 U/μL
• Fidelity (vs. Taq): 25 X
• Format: Kit
• No. of Reactions: 1000 Reactions
• Reaction Format: Separate components
• Reagent Type: Reverse Transcription
• Size (Final Product): 7 kb
• Technique: Reverse Transcription
• No. of Tests: 1000 Reactions
• Quantity: 1000 Reactions

For Research Use Only. Not for use in diagnostic procedures.