High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

Overview
Brand: ABI Applied Biosystems

Manufacturer Part Number: 4368813

KIT, HIGH CAPACITY CDNA RT1000 reactions

UNSPSC: 41106313

Code: 50

Additional Details:
Additional Details: Weight: 0.50000kg



Product Code. 10186954

Quantity Price
1 £ 950.0 / 1000 reactions
EU Stock 103
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

For Use With (Application) cDNA Reverse Transciption, RT-PCR (Endpoint), Real-Time PCR
Format Separate Components
Includes 2 × 1mL of 10X RT Buffer; 2 × 1mL of 10X RT Random Primers; 1 × 1mL of 25X dNTP Mix (100mM); 1 × 1mL of MultiScribe Reverse Transcriptase (50U/μL)
Quantity 1000 reactions
Sample Type RNA
Storage Requirements Store between -15deg. to -25deg.C
Temperature (Metric) Operating 37°C

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in single 20μL reaction. Reactions can be scaled up to 100μL to generate 10μg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

  • Linear target amplification for real-time PCR
  • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
  • Ten-fold greater dynamic range than other kits
  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

  • Extensively tested with a variety of templates
  • Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA

PCR & Real-Time PCR, Reverse Transcription