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Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation

£156.00 - £9200.00

Specifications

For Use With (Application) Hot Start PCR, PCR, PCR and Real-Time PCR, Routine PCR
Overhang 3'-A
Size (Final Product) 5kb or less
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Products 7
Product Code Brand Includes Quantity Price Quantity & Availability  
Product Code Brand Includes Quantity Price Quantity & Availability  
10026054 View Documents Applied Biosystems™
N8080240
AmpliTaq Gold DNA Polymerase (250 units) and Buffer I. 250 units £156.00
Each View alternative products
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EU Stock 24 Please to purchase this item. Need a web account? Register with us today!
10429125 View Documents Applied Biosystems™
N8080246
Contains AmpliTaq Gold DNA Polymerase (1000 units) and Buffer I. 1000 units £516.00
Each View alternative products
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EU Stock 3 Please to purchase this item. Need a web account? Register with us today!
10096094 View Documents Applied Biosystems™
N8080258
5 boxes of AmpliTaq™ Gold DNA polymerase, for a total of 25,000 units (25 x 1000 units). Each box contains 5 tubes x 200μL each of AmpliTaq Gold DNA Polymerase (at 5U/μL), 1000 units total per tube. Each box also contains 5 tubes x 1.5mL of Buffer I (100mM Tris-HCl, pH 8.3, 500mM KCl, 15mM magnesium chloride, 0.01% (w/v) gelatin). 25 x 1000 units £9200.00
25000 units View alternative products
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EU Stock 1 Please to purchase this item. Need a web account? Register with us today!
10229164 View Documents Applied Biosystems™
N8080244
12 tubes x 50μL each of AmpliTaq Gold DNA Polymerase (at 5U/μL, 250 Units total per tube), 12 x 1.5mL of Buffer I (100mM Tris-HCl, pH 8.3, 500mM KCl, 15mM magnesium chloride, 0.01% (w/v) gelatin). 12 x 250 units £1400.00
3000 units View alternative products
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EU Stock 21 Please to purchase this item. Need a web account? Register with us today!
10289814 View Documents Applied Biosystems™
N8080248
5 tubes x 200μL each of AmpliTaq Gold DNA Polymerase (at 5U/μL), 1000 units total per tube. The enzyme also ships with 20 tubes x 1.5mL of Buffer I (100mM Tris-HCl, pH 8.3, 500mM KCl, 15mM magnesium chloride, 0.01% (w/v) gelatin) 5 x 1000 units £2080.00
5000 units View alternative products
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EU Stock 8 Please to purchase this item. Need a web account? Register with us today!
10289204 View Documents Applied Biosystems™
N8080246
Contains AmpliTaq Gold DNA Polymerase (1000 units) and Buffer I. 1000 units This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance
N/A View alternative products
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10036054 View Documents Applied Biosystems™
N8080242
6 tubes x 50μL each of AmpliTaq Gold DNA Polymerase (at 5U/μL, 250 units total per tube), 6 x 1.5mL of Buffer I (100mM Tris-HCl, pH 8.3, 500mM KCl, 15mM magnesium chloride, 0.01% (w/v) gelatin). 6 x 250 units This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance
N/A View alternative products
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Description

Description

Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield
AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

AmpliTaq Gold 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold DNA Polymerase, AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Supplied 360 GC Enhancer works through challenging GC-rich templates.

  • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
  • Time-released thermal activation improves sensitivity in low copy number amplifications
  • Successful multiplex PCR saves time and reagents
  • Includes GeneAmp 10X PCR Buffer I containing 15mM MgCl2

Hot Start PCR, PCR, PCR and Real-Time PCR, Routine PCR

Order Info

Shipping condition: Dry Ice

Specifications

Specifications

Hot Start PCR, PCR, PCR and Real-Time PCR, Routine PCR
3'-A
5kb or less

For Research Use Only. Not for use in diagnostic procedures.