Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

Product Code. 10289814

Brand: Applied Biosystems™ N8080248

Description

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

AmpliTaq Gold DNA Polymerase with Buffer I is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. The modified enzyme is provided in an inactive state and upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases as reactants are not wasted in the formation of unintended products.

Features

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp 10X PCR Buffer I with 15 mM MgCl₂
• Hot-start technology reduces risk of biological contamination

Notes

• For better performance AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation.
• When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.

Order Info

Shipping condition: Dry Ice

Specifications

• Concentration: 5 U/μL
• Content And Storage: • AmpliTaq Gold DNA Polymerase (5 U/μL), 5 x 200 μL
  • 10X PCR Buffer I (with MgCl₂), 20 x 1.5 mL
  
  Store at -15°C to -30°C.
• Format: Tube
• GC-Rich PCR Performance: Low
• Polymerase: AmpliTaq Gold DNA Polymerase
• Reaction Speed: Standard
• Exonuclease Activity: 5' - 3'
• Product Type: DNA Polymerase With Buffer I
• Quantity: 5 x 1,000 units
• Shipping Condition: Dry Ice
• For Use With (Application): Hot-start PCR
• Fidelity (vs. Taq): 1X
• Hot Start: Built-In Hot Start
• No. of Reactions: 4000 Reactions
• Overhang: 3'-A
• Reaction Format: Separate Components
• Size (Final Product): 5 kb or less
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.