AmpliTaq™ 360 DNA Polymerase

Amplifies vast range of DNA sequence contexts when used with enhanced AmpliTaq 360 Buffer and 360 GC Enhancer

Overview
Brand: ABI Applied Biosystems

Manufacturer Part Number: 4398897

25000UNIT AMPLITAQ 360 DNA POLYMERASE

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.50000kg



Product Code. 10330985

Quantity Price
1 £ 5550.0 / 25000 units
Estimated Shipment date
from Supplier 07-12-2016
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Exonuclease Activity 5' - 3'
Fidelity 1 X
GC-Rich PCR Performance High
Includes AmpliTaq Gold 360 DNA Polymerase is supplied at 5U/μL with 10X AmpliTaq Gold 360 PCR Buffer, 25mM M2Cl2, and 360 GC Enhancer. Typically, 1.5mL of each of AmpliTaq Gold 360 Buffer, 25mM MgCl2, and 360 GC Enhancer is supplied with each 250U of enzyme. Storage Buffer Enzyme is supplied in 20mM Tris, pH 9.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% (w/v) Tween 20, 50% (v/v) glycerol. Source: Recombinant, modified form of the Thermus aquaticus DNA polymerase gene expressed in E. coli. All solutions are prepared in deionized, filter-sterilized water.
Overhang 3'-A
Polymerase AmpliTaq Gold™ 360 DNA Polymerase
Quantity 25 x 1000 units
Size (Final Product) 5 kb or less
Storage Requirements When stored at -15 to -25 °C, this product maintains performance at least until the expiration date on the tube label. Before use, thaw frozen components to room temperature and mix well. Product is guaranteed up to 12 months as indicated by the expiration date.

When used with enhanced 360 Buffer, ultra-pure enzyme, reduces false positive results, amplifies low-level target sequences, and promotes amplification of variety of templates including those from bacterial and human genomes for excellent specificity, reproducibility and yield across broad range of targets. Compared to original AmpliTaq DNA polymerase, AmpliTaq 360 polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation.

  • Reduces false positive results
  • Amplifies low-level target sequences
  • Combined with proprietary AmpliTaq 360 Buffer Kit, promotes amplification of variety of templates including those from bacterial and human genomes
  • Optimized for the broadest range of targets–from everyday to challenging
  • Unmatched sensitivity and yield
  • Market-leading GC enhancer for robust amplification of GC-rich sequences
  • Achieves highest quality sequencing data
  • Less waste, sustainable packaging

See user's manual or package insert for limited label license, and trademark information.

PCR, PCR and Real-Time PCR, Routine PCR