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  7. Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG 

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

Product Code. 10575385 Shop All Applied Biosystems Products
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10575385 1250 Reactions 1250 reactions
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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27970” to get your promotional price
Product Code. 10575385 Supplier Applied Biosystems™ Supplier No. 4366073
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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27970” to get your promotional price

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Template and primers

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

  • Results in 40 minutes for 40 cycles of PCR in a 20 μL reaction volume when using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems
  • The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup
  • A proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments
  • Contains everything needed for TaqMan-based qPCR amplification and detection in a single-tube format
  • TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR
  • Compatible with TaqMan Gene Expression assays
  • Store at 2°C to 8°C

Ordreinfo

Guaranteed minimum shelf life is 60 days.

TRUSTED_SUSTAINABILITY

Tekniske data

Concentration 2X
Content And Storage This 5-pack contains five kits, each containing 2 tubes (1.25 ml each) of 2X TaqMan™ Fast Universal PCR Master Mix.

Store at 2°C to 8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq DNA Polymerase
Reaction Speed Fast
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Passive Reference Dye ROX (Pre-mixed)
Product Line AmpErase, TaqMan
Product Type Fast Universal PCR Master Mix
Quantity 1,250 reactions
Sufficient For 250 Reactions
For Use With (Application) Gene Expression, Genotyping, miRNA analysis
Fidelity (vs. Taq) 2 X
No. of Reactions 1250 Reactions
Sample Type DNA (Genomic), cDNA
Volume 1.25 mL
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Frequently Asked Questions (FAQs)

Is the TaqMan Fast Universal PCR Master Mix offered with AmpErase UNG?

The TaqMan Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpEraseUNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan Fast Advanced Master Mix.

For Research Use Only. Not for use in diagnostic procedures.

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