Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

Product Code.	No. of Reactions	unitSize
10311135	500 Reactions	1 set
10637755	5000 Reactions	50mL
10116674	250 Reactions	Each
10575385	1250 Reactions	1250 reactions
10702697	2500 Reactions	1 set

Product Code. 10311135 Supplier Applied Biosystems™ Supplier No. 4366072

Description

Requires

Template and primers

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

• Results in 40 minutes for 40 cycles of PCR in a 20 μL reaction volume when using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems
• The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup
• A proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments
• Contains everything needed for TaqMan-based qPCR amplification and detection in a single-tube format
• TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR
• Compatible with TaqMan Gene Expression assays
• Store at 2°C to 8°C

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

• Concentration: 2X
• Content And Storage: TaqMan™ Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains 2 packs of 2 tubes of 1.25 mL each sufficient for 500 20 μL PCR reactions. Store at 2-8°C
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• Detection Method: Primer-probe
• Format: Tube
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: AmpliTaq DNA Polymerase
• Reaction Speed: Fast
• For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
• Passive Reference Dye: ROX (Pre-mixed)
• Product Line: AmpErase, TaqMan
• Product Type: Fast Universal PCR Master Mix
• Quantity: 500 reactions
• Sufficient For: 500 Reactions
• For Use With (Application): Gene Expression, Genotyping, miRNA analysis
• Fidelity (vs. Taq): 2 X
• No. of Reactions: 500 Reactions
• Sample Type: DNA (Genomic), cDNA
• Volume: 20 μL

Frequently Asked Questions (FAQs)

Is the TaqMan Fast Universal PCR Master Mix offered with AmpErase UNG?

The TaqMan Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpEraseUNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan Fast Advanced Master Mix.

For Research Use Only. Not for use in diagnostic procedures.