AmpErase™ TaqMan™ Fast Universal PCR Master Mix 2X without AmpErase™ UNG

Single-tube format for qPCR amplification and detection

Overview
Brand: ABI Applied Biosystems

Manufacturer Part Number: 4352042

250 RXN FAST UNIVERSAL PCR MASTER MIX (2x1,25ml)AmpErase, UNG 250reactions Store at 4°C

UNSPSC: 41106300

Code: 50

Additional Details:
Additional Details: Weight: 0.03000kg



Requires: Template and primers

Product Code. 10116674

Quantity Price
1 £ 177.0 / Each
EU Stock 603
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Detection Method Primer-Probe Detection
Dye Type ROX (Pre-mixed)
For Use With (Application) Fast Real-Time PCR, Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling
Includes TaqMan Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains two tubes of 1.25mL each sufficient for 250 20-μL PCR reactions.
No. of Tests 250.0
Sample Type DNA (Genomic), cDNA
Shelf Life Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Storage Requirements Store at 2-8°C

Delivers results in 40 minutes for 40 cycles of PCR in a 20μL reaction volume using the Applied Biosystems™ 7900HT and Fast 7500 Fast Real-Time PCR Systems. Optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX™ dye serves as a passive internal reference to normalize non-PCR–related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments.

  • Convenience: Everything needed for TAqMan-based qPCR amplification and detection in a single-tube format, excluding the template and primers, for superior real-time qPCR
  • Compatibility: TAqMan Fast Universal PCR Master Mix (2X), No AmpErase UNG is compatible with TAqMan Gene Expression assays

For use with 7900HT Fast System, 7500 Fast System, StepOnePlus, Fast ModeStepOne, and Fast Mode

Fast Real-Time PCR, Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling