Invitrogen™ ProcartaPlex™ Human Apoptosis Panel, 6plex

Product Code. 17168895

Brand: Invitrogen™ EPX06A15823901

Description

The ProcartaPlex Human Apoptosis Panel 6plex enables examination of the process of programmed cell death by analyzing 6 protein targets in a single well using Luminex xMAP technology.

The ProcartaPlex Human Apoptosis Panel 6plex enables examination of the process of programmed cell death by analyzing 6 protein targets in a single well using Luminex xMAP technology. Simultaneous detection of apoptosis-related proteins along with GAPDH, a housekeeping protein, can help with research of cancer, stroke, Alzheimer's disease, and autoimmune diseases. The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup. It is not combinable with simplexes or other panels.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit our ProcartaPlex Immunoassays page for more information and available products.

Target list [bead region]: Bcl-2 [57], caspase-3 (active) [44], cleaved PARP [28], cytochrome C [54], GAPDH [12], p53 [52]

About ProcartaPlex assays for the Luminex platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.

• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Specifications

• Product Type: Multiplex Panel
• Quantity: 96 Tests
• Species: Human
• Combinability: Non-Combinable
• Assay Sensitivity: See Certificate of Analysis
• Assay Range: See product documentation
• Format: Multiplex Kit
• For Use With (Equipment): Luminex™ Instruments
• Interassay CV: Less than 15%
• Intraassay CV: Less than 15%
• Multiplex Size Plex No.: 6
• Product Line: ProcartaPlex™
• Sample Type: Cell Lysates
• Sample Volume: Cell Lysate: 25 μL (prediluted 1:10)
• Content And Storage:

  • 2 vials Human Standard Mix 12 (lyophilized)
  • 2 vials Human Caspase-3 Standard (lyophilized)
  • 1 vial Capture Bead Mix (1X)
  • 1 vial Biotinylated Detection Antibody Mix (1X)
  • 1 bottle Reading Buffer (1X)
  • 1 bottle Wash Buffer (10X)
  • 1 bottle Streptavidin-PE (1X)
  • 1 bottle Cell Lysate Assay Diluent (1X)
  • 8-tube strip
  • Adhesive film
  • Flat bottom 96-well plate, black
  
  Store at 2°C to 8°C.
  
  

• Shipping Condition: Wet Ice
• Research Area: Apoptosis

For Research Use Only. Not for use in diagnostic procedures.